Abstract
Foods are often contaminated by multiple foodborne pathogens, which threatens human health. In this work, we developed a microfluidic biosensor for multiplex immunoassay of foodborne bacteria with agitation driven by programmed audio signals. This agitation, powered by the vibration of a speaker cone during music playing, accelerated the mass transport in the incubation process to form bacterial complexes within 10 min. Immunoassay reagents of the two target bacteria (Escherichia coli O157:H7 and Salmonella typhimurium) were preloaded into the corresponding fore-vacuum storage chamber on the chip, and released to participate in the subsequent immune analysis process by piercing the chambers. All the detection processes were integrated into a single microfluidic chip and controlled by a smartphone through Bluetooth. Under selected conditions, wide linear ranges and low limits of detection (LODs < 2 CFU/mL) were obtained, and real food samples were successfully determined within 30 min. This biosensing method can be extended to wide-ranging applications by loading different recognizing reagents.
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