A Microfluid Fiber Device for Trace Detection of Aggregation Induced Emission Molecules

Abstract

An ultra-sensitive microfluid device for detecting aggregation of induced emission molecules is proposed and demonstrated. The minimum detectable concentration is greatly reduced by use of a micromachined fiber tip and a liquid core waveguide. The fiber tip is a tapered-microlens structure with optimized shape parameters. The liquid waveguide is formed with low refractive index Teflon capillary. The dependence of photoluminescence intensity on the concentration change is tested with the device. The limit of detection is <inline-formula xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink"> <tex-math notation="LaTeX">$0.316 \boldsymbol {\mu }\text{M}$ </tex-math></inline-formula> , the volume of the sample solution used is only <inline-formula xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink"> <tex-math notation="LaTeX">$0.649 \boldsymbol {\mu }\text{L}$ </tex-math></inline-formula> and the minimum detectable concentration is down to 100 nM. It is a valuable result obtained with fiber spectrometer (a CCD-type machine), better than that obtained with traditional fluorospectro photometer (a photomultiplier tubes-type one). The reversibility and stability are also testified through experimental method. Together with the compact size, small sample volume and high sensitivity, the durable microfluidic sensing device has great potential for biosensing applications.