Abstract
Abstract A microcomputer program (in BASIC, e.g., for SPECTRUM ZX+) is proposed for the calculation of final RF values obtained under conditions of stepwise gradient. After introduction of RF values of sample components obtained for several isocratic runs, the microcomputer calculates the RF values for any gradient program. Good agreement of calculated and experimental RF values was obtained. The stepwise gradient development was carried out using a sandwich chamber with a glass distributor; the eluent fractions according to a chosen stepwise program were introduced directly under the distributor with a micropipette.
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