Abstract
The subclasses of high-density lipoprotein (HDL) labeled with NBD C6-ceramide have been rapidly separated by microfluidic chip electrophoresis and detected by use of laser-induced fluorescence. The subclasses HDL2 and HDL3 were separated in 4 min. Results showed that the HDL2/HDL3 ratio for patients with CHD was much lower than that for healthy subjects. This method could meet the demand for clinical examination of HDL subclasses.
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