Abstract
A method for the micro amperometric determination of thiol-groups as small as from 0.1 to 1.0 μ mole, corresponding to 10–100 μ g of cysteine, was devised. The thiol-group was titrated in ammoniacal solution in the stream of nitrogen with silver nitrate solution, using an ultramicroburette. The course of the titration and the end point was detected amperometrically using platinum vibrating electrode. The method was suitable for the determination of a minute amount of thiol-groups, especially those found in plant tissues, which used to contain such substances as might otherwise disturb the determination. Contents of acid-soluble thiol compounds in etiolated mung bean seedlings at various stages of growth were determined by the method described here.
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