Abstract
Micellar liquid chromatography (MLC) is a useful technique for the determination of compounds in biological samples. Here, a sensitive procedure has been developed for the determination of carbaryl, a phenyl-N-methylcarbamate, and its main metabolite, 1-naphthol, using a C18 column (150 mm × 4.6 mm) and fluorescence detection. After performing optimization studies with an interpretative strategy, the selected mobile phase was sodium dodecyl sulfate (SDS) 0.15 M – 6% (v/v) pentanol - NaH2PO4 0.01 M buffered at pH 3. The validation studies include linearity (r2 > 0.9999), limits of detection and quantification, (0.0325 and 0.1 µM for carbaryl; 0.125 and 0.350 µM for 1-naphthol, respectively), intra- and inter-day precisions below 4%, and robustness parameters below 5%, all of which are adequate for the quantification of these two compounds in urine and serum samples to be directly injected into the chromatographic system.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callMore From: Journal of Liquid Chromatography & Related Technologies
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
