A Method to Improve the Embryogenesis Rate of Banana Somatic Cell Embryogenesis

Shenghe Chang,Haiyan Shu,Wei Sun,Zhengjing Wu,Fei Lin,Jingyi Zhang,Lan Qiao,Qi Zeng

doi:10.4236/ajps.2018.93040

Shenghe Chang, Haiyan Shu + Show 6 more

Open Access

https://doi.org/10.4236/ajps.2018.93040

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Abstract

Getting embryogenic callus is the first step for banana transformation. Enhancing embryonic-callus-rate is important for banana molecular breeding. In this paper, WIND1, an AP2/ERF transcription factor was added into the embryonic-callus-inducing medium with poly-Arg and nuclear localization signal (NLS). The embryogenic-callus-induced rate of the immature male florescence cultured on the media containing the recombinant protein Arg9-NLS-WIND1 was significantly higher than that of the immature male florescence cultured on the control media. Medium containing 0.01% of the protein Arg9-NLS-WIND1 had the best effect for inducing the embryogenesis of the immature male inflorescence. On the medium containing 0.01% of the protein Arg9-NLS-WIND1, 11.5% of the immature male flowers can develop embryonic callus. On the medium containing Arg9-NLS-WIND1, the protein might be transferred into cytoplasm of the explants by endocytosis due to the interaction between the poly-Arg transduction domain and the plasma membrane. After it entered the nucleus guided by NLS and bind with the target DNA domain, the somatic cell embryogenesis reactions were initiated and the embryonic callus formed.

Highlights

IntroductionBanana is an important crop and fruit in tropical and subtropical countries
The embryogenic-callus-induced rate of the immature male florescence cultured on the media containing the recombinant protein Arg9-NLSWIND1 was significantly higher than that of the immature male florescence cultured on the control media
Medium containing 0.01% of the protein Arg9-nuclear localization signal (NLS)-WOUND INDUCED DEDIFFERENTIATION 1 (WIND1) had the best effect for inducing the embryogenesis of the immature male inflorescence

Summary

Introduction

Banana is an important crop and fruit in tropical and subtropical countries. Banana production is always hindered because of lacking good varieties. Breeding new banana varieties through transgenic method is inevitable [1]. Some papers about banana transformation had been published, the transgenic method for banana is still a tough work for many laboratories. The main reasons are 1) banana transformation needed embryogenic suspension cell as initial material due to the worrying about chimera, which required much banana embryogenic callus [2]; 2) The embryogenesis rate of banana somatic cells is very low according to the methods used [3] [4]. If banana embryogenesis rate can be improved significantly, banana transformation might become easier

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Results

Conclusion