Abstract
Predicting variations in microbial growth rates (μ) in Nature requires a method that will detect departures from optimal μ, determine the time scale at sub-optimal μ, and measure the restoration or reversibility toward optimal μ. Isotopic flux measurements are equivocal because of uncertainties in their relationship to μ, particularly in non-steady state condictions. After perturbing a natural microbial community and a clone of the phytoplankter Selenastrum capricornutum with two different toxicants, we examined the kinetics of the adenylate energy charge, and followed changes in biomass over time with the total adenylate and chlorophyll a concentrations. We determined that exposure to cadmium resulted in a reduction in μ; reversible within a short time, whereas exposure to the industrial effluent caused an exponential loss of adenylates and complete inhibition of growth. Upon perturbing a microbiol community, the study of adenylate energy charge kinetics detects departures from optimal μ, measures their duration and reversibility.
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