Abstract
BackgroundThe number of cultivated wheat seedlings per unit area allows calculation of plant density. Wheat seedling density provides emergence data and this is useful for improving crop management. The number of wheat seedlings is typically determined by visual counts but this is time-consuming and laborious.ResultsWe obtained field digital images of 1st to 3rd leaf stage wheat seedlings. The seedlings were extracted using an image analysis technique that calculated the coverage degree of the seedlings and the number of angular points of overlapping leaves. The wheat seedling quantity estimation model was constructed using multivariate regression analysis. The model parameters included coverage degree, number of angular points, variety coefficient, and leaf age. Introduction of the number of angular points increased the accuracy of the single coverage degree model. The R2 value was consistently > 0.95 when the model was applied to different varieties, indicating that the model was adaptable for different varieties. As the leaf stage or density increased, the accuracy of the model declined, but the minimum R2 remained > 0.87, indicating good adaptability of the model to seedlings with different leaf ages and densities.ConclusionsThis method is an effective means for counting wheat seedlings in the 1st to the 3rd leaf stages.
Highlights
The number of cultivated wheat seedlings per unit area allows calculation of plant density
Accurate extraction is the basis for measurement of the coverage degree and detection of angular points
Single‐factor coverage degree model The coverage degree of wheat seedlings is highly correlated to their quantity at the 1st–3rd leaf stages
Summary
The number of cultivated wheat seedlings per unit area allows calculation of plant density. Wheat seedling density provides emergence data and this is useful for improving crop management. The number of wheat seedlings is typically determined by visual counts but this is time-consuming and laborious. Wheat yield and quality are affected by planting density [1, 2]. An optimal density of wheat seedlings provides the best canopy structure and yield. Determining the number of wheat seedlings per unit area provides information on seedling emergence and is the basis for subsequent cultivation and management [3]. The number of seedlings is usually determined manually but manual count is laborious. Image analysis techniques have been applied to several aspects of plant production. Common applications include estimation of crop biomass [4], diagnosis of nutritional status [5], analysis of growth [6], monitoring of
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