Abstract
The hydroid Phialidium gregarium can be successfully cultured to sexual maturity at any time of the year and maintained for years in a closed system marine aquarium. Artificial sea water was used in place of natural sea water and was renewed by one-quarter of its volume each 4 weeks, Freshly hatched Artemia nauplii were used as the sole food source. Methods for obtaining colonies of a single genotype from planulae are described. Subculturing pieces from parental stock is accomplished by a new method. A new technique for removing hydroid colonies from their substrate for histological study is described.
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