A method of islet transplantation under kidney capsule in mouse and the evaluation of it's effect

Abstract

Objective In this paper, we introduced a simple and easy way to do islet transplantation under kidney capsule in mouse, and the effect evaluation of it’s blood glucose, oral glucose tolerance and histological stain after transplantation. Methods BALB/c mice are used in the experiment.The islets were harvested by reversely perfusion of collagenase V into common bile duct and purification by Histopaque density gradient followed by manual picking. After a simple culture, the islets were transplanted under the kidney capsule of diabetic BALB/c mice using a gel loading tip. Then the blood glucose and the oral glucose tolerance test were tested regularly.The kidney was cut at the 10th day after the islet transplantation.The HE and immunofluorescence staining of islet under kidney capsule were also done to evaluate the islet morphology and function. Results We get 1 063± 122 islets, or 1 259.72±107.19 IEQ from four mice at one time.The level of blood glusoce turned down to normal in 24 h after the islet transplantation.Oral glucose tolerance test(OGTT) results showed that the blood glucose levels of mice after islet transplantation were significantly lower than those of diabetes(P< 0.01).And the results of immunofluorescence staining showed that the activity of islets was good[(92.8±2.6)%]. Conclusion The method of the experiment is simple, and the islet activity is good after transplantation, which make a good foundation for the further research on drug screening for islet transplantation. Key words: BALB/c mouse; Islet transplantation; Kidney capsule; Diabetes