Abstract
SUMMARY A gas chromatographic system for the quantitative analysis of blood-gases has been constructed. The system incorporates a simplified equilibration blood-gas extraction and injection unit, and adsorption columns linked in parallel. Eighty μl. of blood is required for each analysis. The time required to carry out duplicate analyses is 8 minutes. Blood oxygen, carbon dioxide and nitrous oxide contents are determined using peak height measurements. Calibration for carbon dioxide is carried out by using carbonate solution, for oxygen by using room air, and for nitrous oxide by using cylinder nitrous oxide. Reproducibility studies were carried out by analyzing one blood sample ten times: coefficients of variation for oxygen, carbon dioxide and nitrous oxide were, in each case, approximately 0.5 per cent. Comparisons of oxygen and carbon dioxide contents of twelve blood samples were simultaneously determined by the gas chromatographic method and by the Van Slyke manometric method. There was close agreement between the two methods; the coefficients of correlation of the respective estimations were +0.999 in the case of oxygen and +0.995 in the case of carbon dioxide.
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