Abstract

A method has been developed for examining mitosis in the leaf meristem of graminaceous plants using perennial ryegrass (Lolium perenne L.), annual ryegrass (Lolium rigidum Gaud.), hexaploid wheat (Triticum aestivum L. em Thell.), oats (Avena sativa L.) and barley (Hordeum vulgare L.). The method consistently gives a high frequency of mitotic stages and appears to have advantages over root-tip methods for examining somatic chromosomes in certain genetic and breeding studies.

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