Abstract
Most methods developed to transform plants either require tissue culture to regenerate a whole plant from transformed tissue or the transformation of reproductive tissue. These approaches are not applicable to vegetatively propagated crops or crops that can not be tissue-culture propagated. We have modified a procedure that was developed to transform zygotic meristems for use with whole plant meristems. We developed a method to introduce DNA via electrophoresis into the cells of axillary meristems of whole plants growing in soil. About half of the treated meristems developed into shoots. Of those shoots, up to half had some level of GUS marker gene expression. We were able to transform Cercis, Chrysanthemum, Capsicum, and Prunus. In Capsicum, the GUS gene was inherited.
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