Abstract
A modification of the Schmidt-Thannhauser procedure for isolating DNA coupled with a new method for DNA digestion and an adaptation of the Berenblum and Chain method for phosphorus determination have been shown to be suitable for use in studying the incorporation of inorganic P 32 into leukocyte DNA. With these techniques tracer doses as small as 2 μc/kg are permitted. Peak tagging of rabbit leukocytes was found to occur on the fourth day following injection. Disappearance of the label through the tenth day was virtually exponential.
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