Abstract

A method has been devised for the reconstruction of three-dimensional models from electron micrographs. Photographic enlargements of electron micrographs of consecutive serial sections are made on lantern slides. The density of each of the transparencies is carefully regulated so that the uppermost section of the series is the least dense and the lowermost section the most dense. The lantern slides are then stacked. Each member of the series is affixed to the one beneath it with rubber cement. The stack of lantern slides is placed on an illuminated stand for the preparation of two photographs, one in either direction from the horizontal. The prints then constitute a stereoscopic pair, which are then mounted for viewing with the stereoscope.

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