Abstract
Analytical methods have been developed for the rapid determination of trace lipid-soluble organic halogenated compounds in biological materials. Biological samples are extracted with a methanol-toluene mixture and, after partitioning with water to remove the inorganic halogens, the resulting toluene layers are assayed for their halogen content. A specifically designed instrument is described for the determination of total halogens (Cl, Br, and I) and the bromine content of these toluene extracts. Lipid-soluble organic halogen concentrations down to 1 μg/g wet weight of tissue were routinely assayed by the methods described.
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