Abstract
In the fall of 1931, while working with male rabbits in an endeavor to utilize them for the Aschheim-Zondek method of diagnosing pregnancy, we were astonished to find that, when ten male rabbits, each 3 months old, were injected with 10 cc. of the first morning urine from women in the last two months of pregnancy, the testicles in five of them became enlarged and differed microscopically from those of the other five, when the rabbits were killed forty-eight hours after injection. In five animals, the testicles were enlarged and congested, and the vessels on the surface were engorged and dilated; when studied microscopically they showed increased cellularity and vascularity and beginning spermatogenesis, as evidenced by the formation of spermatogonia and spermatocytes, features that were not seen in the testicles of the other five animals. In no case, however, were spermatids or sperm cells present. Since the urine was from
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