Abstract
Henry and Housewright1 described a manometric method for the assay of penicillinase which was based on the formation of penicilloic acid from penicillin in the presence of this enzyme. It has been found that a convenient way of following this reaction without the use of a manometric apparatus is to estimate penicilloic acid iodometrically. The uptake of iodine by penicilloic acid is not stoichiometric, 6–9 equivalents of iodine being absorbed per mole of penicilloic acid according to conditions2. However, with a standardized procedure, reproducible results can be obtained.
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