Abstract
A method is presented for determination of the proportions of T4 messengers from the rII region and region D by hybridization competition. The proportions are defined by competition plateaus obtained by comparing RNA from deletion mutants with RNA from wild-type infections. The validity of the method has been verified by mixed competitor analysis, analysis of initial slopes and tests for hybridization specificity. The procedure has been adapted as a preparative step in the isotopic purification of specific messengers. In addition, the end points of several extended rII deletions have been defined, and the gene for acriflavine resistance has been localized at the right end of region D.
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