Abstract
Varroa destructor is one of the most devastating ectoparasites of the honey bee, Apis mellifera, worldwide. Given that V. destructor has very low survivability and exhibits no successful reproduction away from their natural environment and host and that the availability of mites for experimental purposes is limited by seasonality, several protocols of mite rearing under laboratory conditions have been developed. However, only one of these rearing systems has been able to yield a fertile second generation with a low mite survival. The aim of this study was to develop a semi-field rearing method to obtain mites of known age and life cycle that can be maintained through several generations. We registered and compared survival and reproductive parameters of mites of controlled age during four generations (P, F1, F2, F3) and evidenced no significant differences between these mite groups for these life-history traits. With present results we demonstrate that it is possible to successfully produce a third generation of mites under semi-field conditions. This study brings useful information about key conditions for the proper reproduction of mites in a controlled rearing system and provides a potential standardized method for V. destructor research, especially for host-parasite interaction experiments.
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