Abstract
Resting spores of insect pathogenic fungi (Zygomycetes: Entomophthorales) were extracted from soil with a method utilizing discontinuous density gradients. Combinations of CaCl 2, surcrose, and glycerol, or these solutions alone, yielded high recoveries of Conidiobolus thromboides resting spores. The extracting solutions yielding the maximal number of spores of both C. thromboides and Entomophaga grylli were composed of 2.5 or 3 m sucrose and 7 m CaCl 2. Average recovery of resting spores from artificially infested soil was 79.1%, with a range of 59.4–87.5%. Resting spores were also recovered using this method from field-collected soil.
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