Abstract
A method was developed for rearing the egg larval parasitoid, Biosteres arisanus (Sonan) in the laboratory. Papaya fruits were infested with Dacus dorsalis (Hendel) eggs and exposed to field populations of B. arisanus females. Infested fruits were held in the laboratory, and the parasitoids that emerged were used as the founders for laboratory rearing. Adaptation to reproduction under laboratory conditions occurred between the first and the sixth generations. Using the described procedures, B. arisanus was successfully reared through >30 generations in the laboratory. More than 736,000 parasitoids were produced with a self ratio (3:2) of 2.2:1 and mean host parasitism of 37%.
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