Abstract
A method is devised for the separation of mevalonolactone (MVL) from hydroxymethylglutarate (HMG) in the assay of HMG-CoA reductase activity. The main steps in the procedure consist of absorbing the reaction mixture on the bottom part of a rectangular filter paper and selectively transfering the MVL into the top part of the paper by upward elution with toluene. Under the experimental conditions deseribed, MVL is recovered in an yield of approximately 60%, with little contamination with HMG. Among the advantages of the method are that it involves simple and very few manipulations, no internal standard is required to calculate the recovery of MVL, and simultaneous analyses of a large number of samples are possible.
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