Abstract
Pesticide concentration in serum must be measured in order to determine the proper treatment for patients with pesticide poisoning. We previously investigated the effectiveness of a screening method for fat-soluble pesticides by HPLC equipped with photo-diode-array detection (HPLC-DAD) and found that the method was effective in emergency medical units. In the present study, we investigated the effectiveness of a method for quantitative analysis for these fat-soluble pesticides. The concentrations of all pesticides were proportional to the peak area up to 100 μg/ml (injection amount : 1 μg), and the recovery ratio was excellent. This method was applied to three actual cases of acute poisoning, and we were able to give important advice to the doctor according to the concentration of the pesticides in the serum. The screening of pesticides by HPLC-DAD was confirmed by GC-MS.
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