Abstract
A method for quantitation of the abnormal lipoprotein of cholestasis, LP-X, is described. After incubation of the serum with labelled free cholesterol, LP-X is separated from the normally occurring lipoproteins by electrophoresis in agar. By liquid scintillation counting the percentage of free cholesterol present in LP-X is determined. From the serum level of free cholesterol, the amount of free cholesterol in LP-X is calculated. The composition of lipids and proteins in LP-X has previously been found to be fairly constant, and from its content of free cholesterol the amount of LP-X may be calculated.
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