Abstract

To determine phytate phosphorus content in the diet, the WHEELER and FERREL method was modified.Phytate phosphorus was extracted with 3% TCA and precipitated with 0.582% FeCl3 as the Fe4 phytate. The precipitate was washed twice with 3% TCA, twice with N/6 HCl and once with distilled water. Iron content of the precipitate was determined colorimetrically. Phytate phosphorus content was calculated from this value.Phytate phosphorus content determined by the WHEELER and FERREL method was largely affected by calcium phosphates in the diet. However, by the modified method, phytate phosphorus content was able to be determined without being affected by calcium phosphates in the diet.Phytate phosphorus content in major feed ingredients was also determined satisfactory by the modified method.

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