Abstract
This series of papers describes methods for measuring solute partition coefficients between membrane suspensions and water, and applies these methods to the system dimyristoyl lecithin: water. Knowledge of partition coefficients is relevant to analyzing permeability coefficients, probing membrane structure, and evaluating permeation models. The theoretical section of this first paper derives equations to correct measured partition coefficients for the effects of trapped water in the precipitate, tritium exchange with lipids or membranes and nonsolvent water adjacent to membranes. The experimental section presents methods for measuring partition coefficients in liposomes of dimyristoyl lecithin, including techniques for minimizing effects of counter drift and quenching on double-label radioactive counting. These methods offer advantages in measuring membrane: water partition coefficients that are not high.
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