A method for in vivo tracking of mesenchymal stromal cells after intrapancreatic delivery

Abstract

Background and aim. The intrapancreatic injection of mesenchymal stromal cells may be a viable delivery route for experimental therapy in type 1 diabetes. Adequate in vivo cell imaging is important to evaluate the treatment efficiency, the fate of the transplanted cells, and the mechanisms of the effects observed. Here, we present a technique for delivering these cells into the mouse pancreas and tracking them using fluorescent near-infrared quantum dots and in vivo imaging. Methods and results. Bone marrow-derived mesenchymal stromal cells isolated from NOD mice were cultured and labeled with Qdots 800 nanocrystals, before being injected in the pancreas of pre-diabetic mice. In vivo analysis (IVIS Spectrum system) showed that the cells were successfully injected and remained localized in the pancreas for at least 24 hours. Conclusions. Labeling of mesenchymal stromal cells with Qdots 800 nanocrystals is a reliable method for in vivo cell tracking, after local delivery in the pancreas.