Abstract
A microorganism capable of producing glyoxylic acid from glycolic acid was obtained by enrichment culture with medium containing 1,2-propanediol. It was identified as a member of the genus Alcaligenes. The maximal activity of the enzyme responsible for glyoxylic acid production by this strain was obtained by culturing at pH 5–6 for 3 days. The optimum conditions of cell-reaction for accumulating high concentration of glyoxylic acid were investigated using lyophilized cells of 3 day cultivates: Approximately 0.65 M glyoxylic acid was produced from 1.0 M glycolic acid by incubating at 20°C for 15 days in 1.0 M TES-NaOH buffer, pH 7.0. In addition, the production of glyoxylic acid was improved to 0.95 M by keeping the reaction pH at neutral region. The method proposed here is more advantageous for glyoxylic acid production than enzymatic methods.
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