Abstract
In order to improve the utilization of tea seed, microwave/ultrasonic assisted extraction (MUAE) was firstly applied for the extraction of tea seed oil (TSO). The factors with significant effects on oil yield were screened by Plackett-Burman design and then optimized by Box-Behnken design. The oil yield of 31.52% was obtained under optimum extraction conditions of microwave power 440 W, ultrasonic power 550 W, extraction time 38 min, solvent to material ratio 8 mL/g, extraction temperature 70 ℃ and particle size <0.425 mm. No significant differences were found in the fatty acid compositions of oil by MUAE and Soxhlet extraction, while oil by MUAE possessed better physicochemical properties and higher content of bioactive components. Moreover, SEM results illustrated that the structure destruction of tea seed brought about by combining microwave and ultrasonic was the primary driving force for fast extraction. Therefore, MUAE was an efficient method for extracting TSO.
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