Abstract
A fast and low cost method for the isolation of chloroplast DNA (cpDNA), suitable for RFLP analysis, applicable to evolutionary studies in a variety of plant species, is introduced. The method is based on the isolation of chloroplasts in the presence of high salt concentrations and cpDNA isolation using cetyl triammonium bromide (CTAB). The cpDNA preparations obtained can be analysed with restriction endonucleases and are satisfactorily free of nuclear and mitochrondrial DNA contamination. The yield of cpDNA is of the same magnitude as has been described before for other methods
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