Abstract
SummaryA method was developed for testing the effectiveness of germicidal aerosols against air-borne bacteriophages by incorporating the use of an Andersen air sampler containing gelatin plates containing a suitable germicide inactivator. A special chamber 29.25 ft3 in volume was used in which the activity of different aerosols was compared. Standard dosages of bacteriophage particles were sprayed under controlled conditions into the chamber. Immediately following the infection, the experimental germicide was fogged into the chamber. Concentrations and quantities were carefully controlled in order to provide an accurate evaluation of the virucidal effects. Recoverable phages were diluted from liquefied gelatin plates and numbers determined by the plaque count technique.A number of preliminary experiments was conducted to establish a standard procedure. In these experiments the most significant information concerning applications and sampling was derived from the phage fall-out data curve for the test chamber.The procedure described under controlled conditions gave consistently satisfactory results which could be repeated.
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