Abstract
A method was developed for determining cis-dichlorodiammineplatinum (II) (CDDP) in plasma and urine by HPLC with direct UV detection. After one ml of plasma or urine was pretreated with Dowex 1-x8, an aliquot (100 μ1) of the treated plasma or urine was subjected to HPLC using a column packed with Hitachi Gel #3013-N. A mobile phase was prepared with 10 mM sodium chloride in water/methanol (85 : 15) and the detection wavelength was 210 nm. There was a linear relationship between peak height and the concentration of CDDP in plasma and urine in a range from 1 μM to 500 μM. The detection limit was 0.5 μM of CDDP in plasma and urine. CDDP in the plasma and urine of a patient receiving CDDP treatment can be analyzed by this method.
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