Abstract
A method for the visualization of lecithin and sphingomyelin is presented. The procedure allows the use of commercially prepared silica gel thin-layer sheets. Phosphomolybdic acid and stannous chloride solutions have been combined with a novel acetone decolorization which circumvents the usual fixing of the silica gel layer prior to color development. The method produces a permanent record which is suitable for densitometry, facilitating quantitative analysis of the lecithin/sphingomyelin ratios.
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