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Abstract
As the gold standard of protein detection, enzyme-linked immunosorbent assay (ELISA) is widely used in medical treatment and biology. Here, we report a digital ELISA method that combines fluorescence-coded magnetic beads with micropore arrays to effectively improve the accuracy of the detection. Fluorescence coded magnetic beads were used as solid support of ELISA, which were modified to specifically capture IL-6 in serum, and then combined with galactosidase to form a sandwich structure. These beads are then mixed with a fluorescent substrate and passed into a microfluidic chip. Under the action of gravity, the beads are trapped and isolated by an array of micropores in the chip. Combined with image recognition technology, the fluorescence intensity of micropores containing enzymes will increase rapidly. By mining image information, the IL-6 content in uremia patients can be detected with high precision.
Highlights
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Summary
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