Abstract
As the gold standard of protein detection, enzyme-linked immunosorbent assay (ELISA) is widely used in medical treatment and biology. Here, we report a digital ELISA method that combines fluorescence-coded magnetic beads with micropore arrays to effectively improve the accuracy of the detection. Fluorescence coded magnetic beads were used as solid support of ELISA, which were modified to specifically capture IL-6 in serum, and then combined with galactosidase to form a sandwich structure. These beads are then mixed with a fluorescent substrate and passed into a microfluidic chip. Under the action of gravity, the beads are trapped and isolated by an array of micropores in the chip. Combined with image recognition technology, the fluorescence intensity of micropores containing enzymes will increase rapidly. By mining image information, the IL-6 content in uremia patients can be detected with high precision.
Highlights
6WDQGDUG FXUYH GUDZLQJ:H XVHG WKH DERYH PHWKRG WR GHWHFW VL[ JURXSV RI ,/ DW NQRZQ FRQFHQWUDWLRQV EHWZHHQ DQG SJP/ DQG UHFRUGHG WKH FRUUHVSRQGLQJ DFWLYH SRUH UDWLRV 7KHQ WKH OHDVW VTXDUH PHWKRG ZDV XVHG WR ILW WKH VWDQGDUG FXUYH EHWZHHQ ,/ FRQFHQWUDWLRQ DQG WKH SURSRUWLRQ RI DFWLYH SRUHV DV VKRZQ LQ )LJXUH DQG WKH ܴଶ YDOXH RI WKH ILWWLQJ OLQH ZDV %\ GHWHFWLQJ WKH SURSRUWLRQ RI DFWLYH SRUHV LQ WKH VHUXP WR EH WHVWHG DQG VXEVWLWXWLQJ LW LQWR WKH VWDQGDUG FXUYH WKH FRQFHQWUDWLRQ YDOXH RI ,/ LQ WKH WHVWHG VDPSOH FDQ EH REWDLQHG.
)LJ 6HOHFW WKH VSRW RI DSSURSULDWH VL]H LQ WKH IOXRUHVFHQFH LPDJH D )OXRUHVFHQFH JHQHUDWHG E\ WKH IOXRUHVFHQW VXEVWDQFH.
DGGHG LQ WKH H[FLWDWLRQ PDJQHWLF EHDG E )OXRUHVFHQFH JHQHUDWHG E\ IOXRUHVFHQFH VXEVWDQFHV LQ PLFURSRUHV F $IWHU.
Summary
:H XVHG WKH DERYH PHWKRG WR GHWHFW VL[ JURXSV RI ,/ DW NQRZQ FRQFHQWUDWLRQV EHWZHHQ DQG SJP/ DQG UHFRUGHG WKH FRUUHVSRQGLQJ DFWLYH SRUH UDWLRV 7KHQ WKH OHDVW VTXDUH PHWKRG ZDV XVHG WR ILW WKH VWDQGDUG FXUYH EHWZHHQ ,/ FRQFHQWUDWLRQ DQG WKH SURSRUWLRQ RI DFWLYH SRUHV DV VKRZQ LQ )LJXUH DQG WKH ܴଶ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
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
