Abstract
The usual metods for cellulose determinations, excepting those in which the material is decomposed by total hydrolysis, are principally methods for producing cellulose preparations. In these the non-cellulosic compounds are tried to be removed as thoroughly as possible without decomposing the cellulose. However, the preparations obtained still contain different non-cellulosic components for which corrections must be made. In the method proposed by the authors a crude cellulose preparation is produced by a relatively mild treatment, and corrections are made for crude lignin and pentosans. The main feature in producing the crude cellulose preparation is shaking the samples with 3N NaOH solution in pressure bottles in a boiling water bath. The method appears to give readable results for fodder materials. For woods the results are evidently too high, especially due to mannan in the crude cellulose.
Highlights
If the material contains starch, the residue in the Gooch crucible should be boiled % hour with 400 ml of 0.05 N HCI solution, filtered etc. Another sample is treated, but instead of being incinerated, it is used for crude lignin determination
It was mentioned above that the protein of the crude cellulose is not hydrolyzable by the strong acid treatment of the lignin determination and practically all of it remains in the crude lignin
The usual metods for cellulose determinations, excepting those in which the material is decomposed by total hydrolysis, are principally methods for producing cellulose preparations
Summary
The developing of a method for determination of a certain substance occurring in plant or animal materials implies the definition of the substance in question as well as the accessibility of pure preparation of it. The chlorine reacts with lignin to form substitution and oxidation products which are soluble in boiling sodium sulfite solution The crude cellulose contains considerable amounts of pentosans and contains I—31—3 % lignin This method, which has been applied to forage crops, is not intended for determining true cellulose. 333) have developed a cellulose determination method on a quite different basis than the above-mentioned authors In their method the cellulose is hydrolysed by a treatment with 80 % sulphuric acid at room temperature and subsequent boiling in diluted acid. Before these hydrolysis treatments the matter to be analysed is boiled with 2 % HCI solution and extracted with ethanol and ether. Salo (11, p. 32) in her fractionation system uses a similar principle
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
