Abstract
During the past decade, Fourier transform infrared (FT-IR) microspectroscopy has been used successfully in many studies to differentiate normal and diseased tissue samples obtained from a variety of organs, including colon, cervix, prostate, and breast [1-4]. In most cases, spectra were collected for a select area of a tissue or IR images were constructed by collecting spectra point-by-point using a mapping stage on an FTIR microscope equipped with a single-element detector. Recently, a new technique has been developed for performing vibrational spectroscopic imaging microscopy using a liquid-nitrogen-cooled focal-plane array (FPA) detector and a step-scanning FT-IR spectrometer coupled to a refractive microscope [5]. With this configuration, equipped with a new 64-pixel x 64-pixel Mercury-Cadmium-Telluride (MCT) FPA detector, it has now become possible to image an 800-um × 800-um area of a specimen without moving the sample.
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