A method and technique for observing the stereo pseudocolor image of phase change of objects

Abstract

A real-time white light stereo pseudocolor encoding method and technique in a microscope is presented, which demonstrated that the phase information of an object is not totally lost in incoherent imaging. The image system is an improved microscope and attached optical elements can be stacked together in the microscope tube, so the structure is compact. The irradiance distribution at the output plane of the microscope is obtained by means of the theory of partially coherent light. At the conditions of that the aperture stop and focal length of condenser are a right magnitude, and the illuminative light source is incoherent or partially coherent, the theoretical analysis indicates that the irradiance distribution at output plane is presented by the stereo pseudocolor image which is characterized by the phase rate-of-change function of input object. A bleached holographic grating as an input object is observed, and its optical parameters are measured directly. Experimental results are discussed, which basically agreed with theoretical analysis.