Abstract
White spot syndrome virus (WSSV) cause great harm in shrimp aquaculture. To understand the impact of viral infection on the shrimp metabolism, we monitored the culture farms of Litopenaeus vannamei and collected the samples on different stages of WSSV infection. The hepatopancreas of shrimp were separated, and then used gas chromatography mass spectrometry to detect the metabolites. Through the mass spectrometric analysis combined with multivariate data analysis, including PCA and OPLS models, metabolism of the shrimp was significantly changed by WSSV infection. The data indicated that in the early stage of WSSV infection, the glycolysis changed significantly, the contents of glucose and lactate increased distinctly. The metabolites of TCA cycle did not show up obviously regularity. The organism of fatty acids showed the same situation with glycolysis. At the early stage of infection, 14 amino acids metabolism were up-regulated, and glycine still increased at later stage of infection and the concentration was increased twice. The data of this study may provide some information to further research of viral disease mechanism.
Highlights
White spot syndrome virus (WSSV) often brought disastrous to shrimp farming industry
The results were similar when white spot syndrome virus infected the L. vannamei in this study; the results indicated that the WSSV-infected shrimps have a changed hepatopancreas metabolite profile
These results of this paper were like some previous research which described a significant increase in plasma glucose concentration of WSSV infected shrimp; they suggested that shrimp infected with virus may need to use glucose as an energy source to maintain of immune response to resist this rapidly replicating virus (Yoganandhan 2003; Galvan-Alvarez et al 2012)
Summary
White spot syndrome virus (WSSV) often brought disastrous to shrimp farming industry. It is very important to learn the mechanisms of virus attacks the host and the shrimp response to WSSV. Hepatopancrea was an important organ of shrimp metabolize. Some research had focused on genes, biochemical and physiological changes related to WSSV infection in shrimpand differentially expressed proteins in the hepatopancrea of shrimp (Yoganandhan 2003; Leu et al 2007, 2013; Li et al 2013) and upregulation and down-regulation proteins were identified. The above studies show that the host’s metabolism will be affected after the WSSV virus invades, and this negative effect will lead to the shrimp’s immune capacity decline or lead to death. To understand the reaction mechanism of WSSV infection, metabolism data were important supplement to the research.
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