Abstract
Background A general problem in biology is whether different types of cells in the same tissue are metabolically different from each other, and whether such differences are important for cellular function. Experiments that can comprehensively measure the cellular metabolome typically require millions of cultured cells and cannot be used with small numbers of rare cells freshly isolated from tissues. In particular, hematopoietic stem cells (HSCs), the blood forming stem cells of the bone marrow, have been intensively studied for decades but their metabolic composition is largely unknown. Results We have developed a method to measure metabolites in small numbers of HSCs. The HSC isolation and mass spectrometry methods have been optimized for maintenance of the metabolome during purification, sensitivity and robustness. About 50 metabolites can be quantified from 10,000 HSCs, covering a wide spectrum of the cellular metabolome. Several metabolic differences exist between HSCs and other bone marrow cells, including restricted progenitors. I am investigating the role of these metabolic adaptations in HSCs. Conclusion
Highlights
A general problem in biology is whether different types of cells in the same tissue are metabolically different from each other, and whether such differences are important for cellular function
We have developed a method to measure metabolites in small numbers of hematopoietic stem cells (HSCs)
About 50 metabolites can be quantified from 10,000 HSCs, covering a wide spectrum of the cellular metabolome
Summary
A general problem in biology is whether different types of cells in the same tissue are metabolically different from each other, and whether such differences are important for cellular function. A metabolic map of hematopoietic stem cells From Metabolism, Diet and Disease 2014: Cancer and metabolism Washington DC, USA.
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