A meta-analysis of the relationship between RARβ gene promoter methylation and non-small cell lung cancer.

Feng Hua,Xuebing Li,Siwei Zhu,Weisan Zhang,Jundong Gu,Nianzhen Fang,Qing-Yi Wei

doi:10.1371/journal.pone.0096163

Abstract

BackgroundHypermethylation of CpG islands in tumor suppressor gene plays an important role in carcinogenesis. Many studies have demonstrated that hypermethylation in promoter region of RARβ gene could be found with high prevalence in tumor tissue and autologous controls such as corresponding non-tumor lung tissue, sputum and plasma of the NSCLC patients. But with the small number subjects included in the individual studie, the statistical power is limited. Accordingly, we performed this meta-analysis to further asses the relationship of methylation prevalence between the cancer tissue and atuologous controls (corresponding non-tumor lung tissue, sputum and plasma).MethodsThe published articles about RARβ gene promoter hypermethyltion were identified using a systematic search strategy in PubMed, EMBASE and CNKI databases. The pooled odds ratio (OR) of RARβ promoter methylation in lung cancer tissue versus autologous controls were calculated.ResultsFinally, eleven articles, including 1347 tumor tissue samples and 1137 autologous controls were included in this meta-analysis. The pooled odds ratio of RARβ promoter methylation in cancer tissue was 3.60 (95%CI: 2.46–5.27) compared to autologous controls with random-effect model. Strong and significant correlation between tumor tissue and autologous controls of RARβ gene promoter hypermethylation prevalence across studies (Correlation coefficient 0.53) was found.Conclusion RARβ promoter methylation may play an important role in carcinogenesis of the NSCLC. With significant methylation prevalence correlation between tumor tissue and autologous of this gene, methylation detection may be a potential method for searching biomarker for NSCLC.

Highlights

Lung cancer, accounting for 100 million deaths and 120 million incidence per year world-wide, is the leading cause of cancer related death[1]
CpG island methylation of tumor suppressor genes promoters have been found to be important for carcinogenesis in many kinds of carcinomas including non-small cell lung cancer (NSCLC)[3]
The inclusion criteria: non-small cell lung cancer patients with histology or cytology conformation; The methylation was detected by methylation-specifec PCR (MSP) or quantitative MSP (q-MSP); The methylation rate in the cancer tissue and autologous controls would be drawn in the original studies included in this metaanalysis

Summary

Introduction

Lung cancer, accounting for 100 million deaths and 120 million incidence per year world-wide, is the leading cause of cancer related death[1]. CpG island methylation of tumor suppressor genes promoters have been found to be important for carcinogenesis in many kinds of carcinomas including NSCLC[3]. Hypermethylation of CpG islands in tumor suppressor gene plays an important role in carcinogenesis. Many studies have demonstrated that hypermethylation in promoter region of RARb gene could be found with high prevalence in tumor tissue and autologous controls such as corresponding non-tumor lung tissue, sputum and plasma of the NSCLC patients. With the small number subjects included in the individual studie, the statistical power is limited. We performed this meta-analysis to further asses the relationship of methylation prevalence between the cancer tissue and atuologous controls (corresponding non-tumor lung tissue, sputum and plasma)

Methods

Results

Conclusion