Abstract
This paper presents a MEMS differential calorimeter with integrated microfluidics for measuring structural transitions of biomolecules in solution. The device features two identical freestanding membranes, resistive temperature sensors and heaters, and a thermopile differential temperature sensor between the two membranes. Integrated with PDMS microfluidic channels and chambers, the calorimeter allows efficient handling and measurements of small volumes (/spl sim/1 /spl mu/J) of liquid samples. During measurements, the temperatures of the liquid sample and reference materials contained in the microfluidic chambers are scanned over a continuous range of temperatures with on-chip temperature control, and the differential thermal response is monitored by the thermopile, thus allowing differential scanning calorimetry (DSC). We have used the device to measure protein unfolding, one of the most important types of biomolecular structural transitions.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
