Abstract
Pore-spanning planar membranes on highly ordered porous silicon substrates were shown to be well suited to monitor the calcium ion mediated fusion of large unilamellar vesicles by means of confocal laser scanning fluorescence microscopy and scanning ion conductance microscopy in real-time.
Highlights
Pore-spanning planar membranes on highly ordered porous silicon substrates were shown to be well suited to monitor the calcium ion mediated fusion of large unilamellar vesicles by means of confocal laser scanning fluorescence microscopy and scanning ion conductance microscopy in real-time
We present a new and versatile fusion assay based on porespanning membranes that can overcome the drawbacks of black lipid membranes (BLMs) and solid supported membranes (SSMs) (Fig. 1)
Micro-BLMs composed of 1,2-diphythanoyl-sn-glycero-3-phosphocholine (DPhPC)/1-palmitoyl-2-oleoyl-sn-glycero-3-phosphoethanolamine (POPE) (2 : 1) doped with 1 mol% Oregon Green DHPE were formed on gold covered highly ordered porous silicon substrates with pore diameters of 1.2 mm
Summary
Pore-spanning planar membranes on highly ordered porous silicon substrates were shown to be well suited to monitor the calcium ion mediated fusion of large unilamellar vesicles by means of confocal laser scanning fluorescence microscopy and scanning ion conductance microscopy in real-time. While the Texas Red fluorescence increases if the vesicle approaches the planar membrane interface, it decreases upon lipid mixing.
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