A member of NF-Y family, OsNF-YC5 negatively regulates salt tolerance in rice

Abstract

NF-Y, a critical transcription factor, binds to the CCAAT-box in target gene promoters, playing a pivotal role in plant development and abiotic stress response. OsNF-YC5, encodes a putative subunit of the NF-Y transcription factor in rice, had an undetermined function. Our research revealed that OsNF-YC5 is induced by high salinity and exogenous abscisic acid (ABA). Subcellular localization studies showed that OsNF-YC5 is nuclear- and cytoplasm-localized. Using CRISPR-Cas9 to disrupt OsNF-YC5, we observed significantly enhanced rice salinity tolerance and ABA-hypersensitivity. Compared to the wild-type, osnf-yc5 mutants exhibited reduced H2O2 and malondialdehyde (MDA) levels, increased catalase (CAT) activity, and elevated OsCATA transcripts under salt stress. Moreover, ABA-dependent (OsABI2 and OsLEA3) and ABA-independent (OsDREB1A, OsDREB1B, and OsDREB2A) marker genes were upregulated in mutant lines in response to salinity. These results indicate that disrupting OsNF-YC5 enhances rice salinity tolerance, potentially by boosting CAT enzyme activity and modulating gene expression in both ABA-dependent and ABA-independent pathways. Therefore, this study provides a valuable theoretical foundation and genetic resources for developing novel salt-tolerant rice varieties.