Abstract
The organic acid content is one of the important factors that determine fruit quality. In this study, one gene encoding a P3B-ATPase (designated as MdMa13) was identified as a determinant of fruit acidity in apple. Subcellular localization indicated that MdMa13 was located in the tonoplast. Overexpression of MdMa13 in apple calli, tomato or apples led to increased malic acid contents. In the transgenic apple calli, the expression levels of seven acid-related genes were higher than that in the wild-type apple calli, including MdMa1, MdtDT, MdDTC1, MdDTC2, MdVHA-A3, MdVHA-D, and MdMa11. The expression level of MdMa10 was lower in transgenic lines than that in wild-type apple calli. Together, these findings implied that MdMa13 has important functions related to apple fruit acidity. The results of this study will strengthen the characterization for the complex molecular regulation of fruit acidity.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have