Abstract
A chlorophyll-deficient mutant of Nicotiana plumbaginifolia Viv. has been regenerated from a pale green sector on a leaf of a plant transgenic for a neomycin phosphotransferase II (NPTII) gene conferring kanamycin resistance. Reciprocal backcrosses with green wildtype plants established a cytoplasmic basis for the mutation to chlorophyll deficiency. In vitro shoots cultured either in darkness or light grew as well as wildtype shoots in darkness, although substantially less than wildtype shoots in light. These shoots are deficient in both chlorophylls a and b, and retained the kanamycin resistance phenotype. The transgenic status of the chlorophyll-deficient, kanamycin-resistant plants was confirmed using the polymerase chain reaction to detect the presence of the NPTII gene and a dot blot assay for the NPTII enzyme activity. The combination of chlorophyll deficiency and kanamycin resistance offers a valuable phenotype for plant cell genetics, e.g., as a universal hybridizer for somatic cell fusion to any wildtype plant cell, especially for chloroplast transfers.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
