Abstract

Pluripotency can be captured in vitro in the form of Embryonic Stem Cells (ESCs). These ESCs can be either maintained in the unrestricted "naïve" state of pluripotency, adapted to developmentally more constrained "primed" pluripotency or differentiated towards each of the three germ layers. Epigenetic protein complexes and transcription factors have been shown to specify and instruct transitions from ESCs to distinct cell states. In this study, proteomic profiling of the chromatin landscape by chromatin enrichment for proteomics (ChEP) is used in mouse naive pluripotent ESCs, primed pluripotent Epiblast stem cells (EpiSCs), and cells in early stages of differentiation. A comprehensive overview of epigenetic protein complexes associated with the chromatin is provided and proteins associated with the maintenance and loss of pluripotency are identified. The data reveal major compositional alterations of epigenetic complexes during priming and differentiation of naïve pluripotent ESCs. These results contribute to the understanding of ESC differentiation and provide a framework for future studies of lineage commitment of ESCs.

Highlights

  • Cells (ESCs) are receptive to differentiation-inducing cues from (ESCs)

  • Naıve ESCs of mice can be derived from pre-implantations proteomic profiling of the chromatin landscape by chromatin enrichment for embryos and can be maintained in fetal proteomics (ChEP) is used in mouse naive pluripotent ESCs, primed pluripotent Epiblast stem cells (EpiSCs), and cells in early stages of differentiation

  • A comprehensive overview of epigenetic protein complexes associated with the chromatin is provided and proteins associated with the maintenance and loss of pluripotency are identified

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Summary

DATASET BRIEF

A Mass Spectrometry Survey of Chromatin-Associated Proteins in Pluripotency and Early Lineage Commitment. The data reveal major calf serum supplemented with Leukemia Inhibitory Factor (LIF),[5,6] which mediate activation of the SMAD and JAK-STAT signaling, respectively.[2] Upon injection into pre-implantation embryos, these cells can contribute to all germ layers compositional alterations of epigenetic complexes during priming and and the germline.[3] The in vitro cultured differentiation of naıve pluripotent ESCs. The data reveal major calf serum supplemented with Leukemia Inhibitory Factor (LIF),[5,6] which mediate activation of the SMAD and JAK-STAT signaling, respectively.[2] Upon injection into pre-implantation embryos, these cells can contribute to all germ layers compositional alterations of epigenetic complexes during priming and and the germline.[3] The in vitro cultured differentiation of naıve pluripotent ESCs These results contribute to the understanding of ESC differentiation and provide a framework for future studies of lineage commitment of ESCs. equivalents of post-implantation pluripotent cells are Epiblast stem cells (EpiSCs), of which the maintenance relies on stimulation of SMAD and ERK signaling by Activin A and FGF2, respectively.[7,8]

EpiSCs are more developmentally
Epigenetic processes play a substantial role in regulation of
Experimental Section
Findings
Conflict of Interest
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