A map of transcriptional heterogeneity and regulatory variation in human microglia.

Abstract

Microglia, the tissue resident macrophages of the CNS, play critical roles in immune defence, development and homeostasis. However, isolating microglia from humans in large numbers is challenging. Here, we profiled gene expression variation in primary human microglia isolated from 141 patients undergoing neurosurgery. Using single cell and bulk RNA sequencing, we identify how age, sex and clinical pathology influence microglia gene expression and which genetic variants have microglia-specific functions using expression quantitative trait loci (eQTL) mapping. We follow up one of our findings using an hIPSC-based macrophage model to fine-map a candidate causal variant for Alzheimer’s disease at the BIN1 locus. Our study provides the first population-scale transcriptional map of a critically important cell for human CNS development and disease.