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Abstract
Injection of sperm extracts triggers Ca 2+ oscillations in mammalian eggs similar to those seen at fertilisation. Here, we show that addition of sperm extracts to sea urchin egg homogenates causes Ca 2+ release and inositol 1,4,5-trisphosphate (InsP 3) production. Furthermore depleting homogenates of phosphatidylinositol lipids using a phosphatidylinositol-specific phospholipase C blocked the sperm extract from causing InsP 3 production and a Ca 2+ rise. A response could be recovered by the addition of phosphatidylinositol 4,5-bisphosphate to either sperm extracts or egg homogenates. These data indicate that sperm extracts contain an InsP 3-generating phospholipase C which may play a role in Ca 2+ release at fertilisation.
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